最佳答案
我想你使用的是wave library,对吧？
原文链接：https://www.f2er.com/python/439224.html文档说：
wave.open(file[,mode])
If file is a string,open the file by that name,otherwise treat it as a seekable file-like object.
这意味着您应该能够做到以下几点：
>>> import wave
>>> from StringIO import StringIO
>>> file_on_disk = open('myfile.wav','rb')
>>> file_in_memory = StringIO(file_on_disk.read())
>>> file_on_disk.seek(0)
>>> file_in_memory.seek(0)
>>> file_on_disk.read() == file_in_memory.read()
True
>>> wave.open(file_in_memory,'rb')
编辑(参见评论)：以防你的问题不只是从内存中读取文件而是从python中完全播放…
一个选项是使用pymedia
import time,wave,pymedia.audio.sound as sound
f= wave.open( 'YOUR FILE NAME','rb' ) # ← you can use StrinIO here!
sampleRate= f.getframerate()
channels= f.getnchannels()
format= sound.AFMT_S16_LE
snd= sound.Output( sampleRate,channels,format )
s= f.readframes( 300000 )
snd.play( s )
while snd.isPlaying(): time.sleep( 0.05 )
[来源：pymedia tutorial(为简洁起见,我省略了他们的解释性意见]